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KMID : 0370220040480040231
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Yakhak Hoeji
2004 Volume.48 No. 4 p.231 ~ p.235
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Determination of Hyperin in Acanthopanax senticosus and A. sessiliflorus by HPLC
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이상현/Lee SH
정하숙/신국현/김박광/Chung HS/Shin KH/Kim BK
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Abstract
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A new high performance liquid chromatographic method was applied for the determination of hyperin in Acanthopanax senticosus and A. Sessiliflorus. The stationar y phase used was μ-Bondapak C18 reverse- phase column and a mobile phase program was a gradient of acetonitrile and distilled water at a flow rate of 1.0 ml/min. Hyperin was detected at 210 nm, and the analysis was successfully carried out within 20 min. Hyperin was detected in the one year- grown and two years-grown stem of A. senticosus (0.47 and 0.13 mg/g, respectively) and A. sessiliflorus (0.14 and 0.03 mg/g, respectively). Hyperin was detected in the main and branch root of A. sessiliflorus (0.30 and 0.09 mg/g, respectively). But there is no detection of hyperin in the main and branch root of A. senticosus.
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